About the Authors

James A. Stapleton

Affiliation Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, United States of America

Jeongwoon Kim

Affiliation Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

John P. Hamilton

Affiliation Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

Ming Wu

Affiliation Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, 48109, United States of America

Luiz C. Irber

Affiliation Department of Microbiology and Molecular Genetics and Department of Computer Science and Engineering, Michigan State University, East Lansing, Michigan, United States of America

Rohan Maddamsetti

Affiliations BEACON Center for the Study of Evolution in Action, Michigan State University, East Lansing, Michigan, United States of America, Department of Integrative Biology, Michigan State University, East Lansing, Michigan, United States of America

Bryan Briney

Affiliations Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America, Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, California, United States of America, International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California, United States of America

Linsey Newton

Affiliation Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

Dennis R. Burton

Affiliations Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America, Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, California, United States of America, International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California, United States of America

C. Titus Brown

Affiliations Department of Microbiology and Molecular Genetics and Department of Computer Science and Engineering, Michigan State University, East Lansing, Michigan, United States of America, Population Health and Reproduction, University of California Davis, Davis, California, United States of America

Christina Chan

Affiliation Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, United States of America

C. Robin Buell

Affiliation Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

Timothy A. Whitehead

taw@egr.msu.edu

Affiliations Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, United States of America, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, Michigan, United States of America

Competing Interests

The authors of this manuscript have read the journal's policy and have the following competing interests: Concepts described in this publication have been protected by US Patent Application number 14947988, “Methods for assembling and reading nucleic acid sequences from mixed populations.” This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: JAS JK JPH RM BB DRB CC CRB TAW. Performed the experiments: JAS JK JPH LN. Analyzed the data: JAS JK JPH MW LCI RM CTB CRB TAW. Contributed reagents/materials/analysis tools: DRB CC CRB. Wrote the paper: JAS JK JPH CRB TAW.