Extracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres

Sejin Han; Yuk Yin Li; Barbara Pui Chan

doi:10.1371/journal.pone.0146928

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Open Access

Peer-reviewed

Research Article

Extracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres

Sejin Han,

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China
⨯
Yuk Yin Li,

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China
⨯
Barbara Pui Chan

* E-mail: bpchan@hku.hk

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China
⨯

Extracellular Protease Inhibition Alters the Phenotype of Chondrogenically Differentiating Human Mesenchymal Stem Cells (MSCs) in 3D Collagen Microspheres

Sejin Han,
Yuk Yin Li,
Barbara Pui Chan

Published: January 13, 2016
https://doi.org/10.1371/journal.pone.0146928

About the Authors

Sejin Han

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China

Yuk Yin Li

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China

Barbara Pui Chan

* E-mail: bpchan@hku.hk

Affiliation Tissue Engineering Laboratory, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, China

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BPC SJH. Performed the experiments: SJH. Analyzed the data: BPC SJH YYL. Contributed reagents/materials/analysis tools: BPC. Wrote the paper: BPC YYL SJH. Financial support: BPC.

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Collagens
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Extracellular matrix
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Protease inhibitors
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Proteases
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Gene expression
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Immunofluorescence staining
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Mesenchymal stem cells
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Cell differentiation
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