Mutations to Less-Preferred Synonymous Codons in a Highly Expressed Gene of Escherichia coli: Fitness and Epistatic Interactions

David J. Hauber; Dennis W. Grogan; Ronald W. DeBry

doi:10.1371/journal.pone.0146375

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Open Access

Peer-reviewed

Research Article

Mutations to Less-Preferred Synonymous Codons in a Highly Expressed Gene of Escherichia coli: Fitness and Epistatic Interactions

David J. Hauber,

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America
⨯
Dennis W. Grogan,

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America
⨯
Ronald W. DeBry

* E-mail: ron.debry@uc.edu

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America
⨯

Mutations to Less-Preferred Synonymous Codons in a Highly Expressed Gene of Escherichia coli: Fitness and Epistatic Interactions

David J. Hauber,
Dennis W. Grogan,
Ronald W. DeBry

Published: January 4, 2016
https://doi.org/10.1371/journal.pone.0146375

About the Authors

David J. Hauber

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America

Dennis W. Grogan

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America

Ronald W. DeBry

* E-mail: ron.debry@uc.edu

Affiliation Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DJH DWG RWD. Performed the experiments: DJH. Analyzed the data: DJH RWD. Contributed reagents/materials/analysis tools: DJH DWG. Wrote the paper: DJH DWG RWD.

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Silent mutation
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Natural selection
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Gene expression
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Mutation
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Cloning
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Mutant strains
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Fitness epistasis
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Plasmid construction
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