A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpressTM

Matthew W. Nowicki; Elizabeth A. Blackburn; Iain W. McNae; Martin A. Wear

doi:10.1371/journal.pone.0146164

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Peer-reviewed

Research Article

A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpress^TM

Matthew W. Nowicki,

Affiliation The Edinburgh Protein Production Facility (EPPF), Wellcome Trust Centre for Cell Biology (WTCCB), University of Edinburgh, King's Buildings, Max Born Crescent, Mayfield Road, Edinburgh, United Kingdom
⨯
Elizabeth A. Blackburn,

Affiliation The Edinburgh Protein Production Facility (EPPF), Wellcome Trust Centre for Cell Biology (WTCCB), University of Edinburgh, King's Buildings, Max Born Crescent, Mayfield Road, Edinburgh, United Kingdom
⨯
Iain W. McNae,

Affiliation The Edinburgh Protein Production Facility (EPPF), Wellcome Trust Centre for Cell Biology (WTCCB), University of Edinburgh, King's Buildings, Max Born Crescent, Mayfield Road, Edinburgh, United Kingdom
⨯
Martin A. Wear

* E-mail: martin.wear@ed.ac.uk

Affiliation The Edinburgh Protein Production Facility (EPPF), Wellcome Trust Centre for Cell Biology (WTCCB), University of Edinburgh, King's Buildings, Max Born Crescent, Mayfield Road, Edinburgh, United Kingdom
⨯

A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpress^TM

Matthew W. Nowicki,
Elizabeth A. Blackburn,
Iain W. McNae,
Martin A. Wear

Published: December 30, 2015
https://doi.org/10.1371/journal.pone.0146164

About the Authors

Matthew W. Nowicki

Affiliation The Edinburgh Protein Production Facility (EPPF), Wellcome Trust Centre for Cell Biology (WTCCB), University of Edinburgh, King's Buildings, Max Born Crescent, Mayfield Road, Edinburgh, United Kingdom

Elizabeth A. Blackburn

Iain W. McNae

Martin A. Wear

* E-mail: martin.wear@ed.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MN MAW. Performed the experiments: MN EAB IWM MAW. Analyzed the data: MN EAB MAW. Wrote the paper: MN EAB MAW.

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