Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

Nathaniel M. Butler; Paul A. Atkins; Daniel F. Voytas; David S. Douches

doi:10.1371/journal.pone.0144591

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Open Access

Peer-reviewed

Research Article

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

Nathaniel M. Butler,

Affiliation Department of Plant, Soils and Microbial Sciences, Michigan State University, East Lansing, Michigan, 48824, United States of America
⨯
Paul A. Atkins,

Affiliation Department of Genetics, Cell Biology and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, 55455, United States of America
⨯
Daniel F. Voytas,

Affiliation Department of Genetics, Cell Biology and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, 55455, United States of America
⨯
David S. Douches

* E-mail: douchesd@msu.edu

Affiliation Department of Plant, Soils and Microbial Sciences, Michigan State University, East Lansing, Michigan, 48824, United States of America
⨯

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

Nathaniel M. Butler,
Paul A. Atkins,
Daniel F. Voytas,
David S. Douches

Published: December 14, 2015
https://doi.org/10.1371/journal.pone.0144591

About the Authors

Nathaniel M. Butler

Affiliation Department of Plant, Soils and Microbial Sciences, Michigan State University, East Lansing, Michigan, 48824, United States of America

Paul A. Atkins

Affiliation Department of Genetics, Cell Biology and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, 55455, United States of America

Daniel F. Voytas

Affiliation Department of Genetics, Cell Biology and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, 55455, United States of America

David S. Douches

* E-mail: douchesd@msu.edu

Affiliation Department of Plant, Soils and Microbial Sciences, Michigan State University, East Lansing, Michigan, 48824, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: NMB. Performed the experiments: NMB. Analyzed the data: NMB. Contributed reagents/materials/analysis tools: PAA DFV. Wrote the paper: NMB PAA DFV DSD.

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Polymerase chain reaction
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Cloning
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Tetraploidy
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Deletion mutation
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Mutation detection
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Potato
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CRISPR
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Genomics
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