About the Authors

Gertjan Kramer

gertjan.kramer@embl.de

Current address: Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

Yvonne Woolerton

Affiliation Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom

Jan P. van Straalen

Affiliation Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

Johannes P. C. Vissers

Affiliation Waters Corporation, MS Technologies Centre, Manchester, United Kingdom

Nick Dekker

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

James I. Langridge

Affiliation Waters Corporation, MS Technologies Centre, Manchester, United Kingdom

Robert J. Beynon

Affiliation Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom

Dave Speijer

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

Auguste Sturk

Affiliation Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

Johannes M. F. G. Aerts

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands

Competing Interests

Authors JPCV and JIL are employed by Waters Corporation. The methods described in the manuscript make use of the following Waters Corporation marketed products: a nanoAcquity system, a Synapt G2 MS mass spectrometer, ProteinLynx GlobalSERVER (PLGS) v2.5, Mass Prep Quantitation standard, and RapiGest SF. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: GK JMFGA AS. Performed the experiments: GK YW JPvS. Analyzed the data: GK ND JPvS JPCV YW DS. Contributed reagents/materials/analysis tools: YW RJB JPvS AS JIL JPCV. Wrote the paper: GK DS ND YW RJB JPvS AS JMFGA JIL JPCV. Provided technical support during the mass-spectrometry measurements and advised on data analysis, but did not have any undue influence in the study design, data collection and analysis, decision to publish, or preparation of the manuscript: JPCV JIL.