Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation

Zhonghua Wang; Akash Bhattacharya; Dmitri N. Ivanov

doi:10.1371/journal.pone.0138780

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Open Access

Peer-reviewed

Research Article

Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation

Zhonghua Wang ,

* E-mail: wangz3@uthscsa.edu

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America
⨯
Akash Bhattacharya,

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America
⨯
Dmitri N. Ivanov

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America
⨯

Identification of Small-Molecule Inhibitors of the HuR/RNA Interaction Using a Fluorescence Polarization Screening Assay Followed by NMR Validation

Zhonghua Wang,
Akash Bhattacharya,
Dmitri N. Ivanov

Published: September 21, 2015
https://doi.org/10.1371/journal.pone.0138780

About the Authors

Zhonghua Wang

* E-mail: wangz3@uthscsa.edu

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America

Akash Bhattacharya

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America

Dmitri N. Ivanov

Affiliation Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: ZW. Performed the experiments: ZW. Analyzed the data: ZW. Contributed reagents/materials/analysis tools: DNI AB. Wrote the paper: ZW DNI.

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Library screening
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Protein interactions
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Breast cancer
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Fluorescence polarization
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Messenger RNA
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NMR spectroscopy
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RNA-binding proteins
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Cancer treatment
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