Application of β-Lactamase Reporter Fusions as an Indicator of Effector Protein Secretion during Infections with the Obligate Intracellular Pathogen Chlamydia trachomatis

Konrad E. Mueller; Kenneth A. Fields

doi:10.1371/journal.pone.0135295

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Open Access

Peer-reviewed

Research Article

Application of β-Lactamase Reporter Fusions as an Indicator of Effector Protein Secretion during Infections with the Obligate Intracellular Pathogen Chlamydia trachomatis

Konrad E. Mueller,

Affiliation Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY, United States of America
⨯
Kenneth A. Fields

* E-mail: Ken.fields@uky.edu

Affiliation Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY, United States of America
⨯

Application of β-Lactamase Reporter Fusions as an Indicator of Effector Protein Secretion during Infections with the Obligate Intracellular Pathogen Chlamydia trachomatis

Konrad E. Mueller,
Kenneth A. Fields

Published: August 10, 2015
https://doi.org/10.1371/journal.pone.0135295

About the Authors

Konrad E. Mueller

Affiliation Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY, United States of America

Kenneth A. Fields

* E-mail: Ken.fields@uky.edu

Affiliation Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KEM KAF. Performed the experiments: KEM KAF. Analyzed the data: KEM KAF. Contributed reagents/materials/analysis tools: KEM KAF. Wrote the paper: KEM KAF.

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Chlamydia infection
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Chlamydia trachomatis
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Chlamydia
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Secretion
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Protein secretion
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HeLa cells
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Secretion systems
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Polymerase chain reaction
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