Paraformaldehyde Fixation May Lead to Misinterpretation of the Subcellular Localization of Plant High Mobility Group Box Proteins

Man-Wah Li; Liang Zhou; Hon-Ming Lam

doi:10.1371/journal.pone.0135033

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Open Access

Peer-reviewed

Research Article

Paraformaldehyde Fixation May Lead to Misinterpretation of the Subcellular Localization of Plant High Mobility Group Box Proteins

Man-Wah Li ,

Contributed equally to this work with: Man-Wah Li, Liang Zhou

Affiliation Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong
⨯
Liang Zhou ,

Contributed equally to this work with: Man-Wah Li, Liang Zhou

Affiliations Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, Guangdong, People’s Republic of China
⨯
Hon-Ming Lam

* E-mail: honming@cuhk.edu.hk

Affiliation Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong
⨯

Paraformaldehyde Fixation May Lead to Misinterpretation of the Subcellular Localization of Plant High Mobility Group Box Proteins

Man-Wah Li,
Liang Zhou,
Hon-Ming Lam

Published: August 13, 2015
https://doi.org/10.1371/journal.pone.0135033

About the Authors

Man-Wah Li

Contributed equally to this work with: Man-Wah Li, Liang Zhou

Affiliation Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong

Liang Zhou

Contributed equally to this work with: Man-Wah Li, Liang Zhou

Affiliations Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, Guangdong, People’s Republic of China

Hon-Ming Lam

* E-mail: honming@cuhk.edu.hk

Affiliation Centre for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MWL LZ HML. Performed the experiments: MWL LZ. Analyzed the data: MWL LZ HML. Contributed reagents/materials/analysis tools: HML. Wrote the paper: MWL LZ HML.

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Metaphase
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Fluorescence recovery after photobleaching
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Cell staining
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DNA-binding proteins
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Paraformaldehyde fixation
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Confocal microscopy
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Chromosome staining
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Arabidopsis thaliana
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