About the Authors

Sílvia Fontenete

sfontenete@fe.up.pt

Affiliations LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark, ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal

Marina Leite

Affiliations Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

Nuno Guimarães

Affiliations LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark

Pedro Madureira

Affiliations Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal, IBMC, Institute for Molecular Biology and Cell Biology, Porto, Portugal

Rui Manuel Ferreira

Affiliations Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

Céu Figueiredo

Affiliations Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, FMUP, Faculty of Medicine of Porto University, Porto, Portugal

Jesper Wengel

Affiliation IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

Nuno Filipe Azevedo

Affiliation LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal

Competing Interests

JW is cofounder of RiboTask ApS, which offer LNA/2’-OMe-RNA probes for RNA targeting. NFA is cofounder of Biomode SA, which develops molecular methods for the rapid detection of microorganisms. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: SF ML JW NFA. Performed the experiments: SF. Analyzed the data: SF ML JW NFA. Contributed reagents/materials/analysis tools: SF ML PM CF RMF JW NFA. Wrote the paper: SF ML NG CF JW NFA.