The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain

Santiago Català; Ana Pérez-Sierra; Paloma Abad-Campos

doi:10.1371/journal.pone.0119311

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Open Access

Peer-reviewed

Research Article

The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain

Santiago Català ,

* E-mail: sangarca@upv.es

Affiliation Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain
⨯
Ana Pérez-Sierra,

Affiliations Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain, Forest Research, Alice Holt Lodge, Farnham, Surrey, United Kingdom
⨯
Paloma Abad-Campos

Affiliation Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain
⨯

The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain

Santiago Català,
Ana Pérez-Sierra,
Paloma Abad-Campos

Published: March 16, 2015
https://doi.org/10.1371/journal.pone.0119311

About the Authors

Santiago Català

* E-mail: sangarca@upv.es

Affiliation Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain

Ana Pérez-Sierra

Affiliations Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain, Forest Research, Alice Holt Lodge, Farnham, Surrey, United Kingdom

Paloma Abad-Campos

Affiliation Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SC PAC APS. Performed the experiments: SC APS. Analyzed the data: SC. Contributed reagents/materials/analysis tools: SC PAC APS. Wrote the paper: SC APS.

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Phytophthora
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Polymerase chain reaction
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Forests
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Plant pathogens
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Surface water
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Species diversity
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Ecosystems
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Spain
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