Histidine 352 (His352) and Tryptophan 355 (Trp355) Are Essential for Flax UGT74S1 Glucosylation Activity toward Secoisolariciresinol

Kaushik Ghose; Jason McCallum; Marva Sweeney-Nixon; Bourlaye Fofana

doi:10.1371/journal.pone.0116248

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Peer-reviewed

Research Article

Histidine 352 (His³⁵²) and Tryptophan 355 (Trp³⁵⁵) Are Essential for Flax UGT74S1 Glucosylation Activity toward Secoisolariciresinol

Kaushik Ghose,

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Jason McCallum,

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Marva Sweeney-Nixon,

Affiliation University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Bourlaye Fofana

* E-mail: bourlaye.fofana@agr.gc.ca

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
⨯

Histidine 352 (His³⁵²) and Tryptophan 355 (Trp³⁵⁵) Are Essential for Flax UGT74S1 Glucosylation Activity toward Secoisolariciresinol

Kaushik Ghose,
Jason McCallum,
Marva Sweeney-Nixon,
Bourlaye Fofana

Published: February 25, 2015
https://doi.org/10.1371/journal.pone.0116248

About the Authors

Kaushik Ghose

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada

Jason McCallum

Marva Sweeney-Nixon

Affiliation University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada

Bourlaye Fofana

* E-mail: bourlaye.fofana@agr.gc.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BF. Performed the experiments: KG JM. Analyzed the data: BF KG JM. Contributed reagents/materials/analysis tools: BF MS. Wrote the paper: BF KG JM MS. Coordination and administration: BF MS.

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Hydrogen bonding
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Oxygen
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Protein structure
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Site-directed mutagenesis
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Flax
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Protein structure prediction
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Protein structure databases
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Mutagenesis
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