FindFoci: A Focus Detection Algorithm with Automated Parameter Training That Closely Matches Human Assignments, Reduces Human Inconsistencies and Increases Speed of Analysis

Alex D. Herbert; Antony M. Carr; Eva Hoffmann

doi:10.1371/journal.pone.0114749

Loading metrics

Open Access

Peer-reviewed

Research Article

FindFoci: A Focus Detection Algorithm with Automated Parameter Training That Closely Matches Human Assignments, Reduces Human Inconsistencies and Increases Speed of Analysis

Alex D. Herbert ,

* E-mail: a.herbert@sussex.ac.uk

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom
⨯
Antony M. Carr,

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom
⨯
Eva Hoffmann

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom
⨯

FindFoci: A Focus Detection Algorithm with Automated Parameter Training That Closely Matches Human Assignments, Reduces Human Inconsistencies and Increases Speed of Analysis

Alex D. Herbert,
Antony M. Carr,
Eva Hoffmann

Published: December 5, 2014
https://doi.org/10.1371/journal.pone.0114749

About the Authors

Alex D. Herbert

* E-mail: a.herbert@sussex.ac.uk

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom

Antony M. Carr

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom

Eva Hoffmann

Affiliation MRC Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, BN1 9RQ, United Kingdom

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: ADH EH AMC. Performed the experiments: ADH. Analyzed the data: ADH EH. Contributed reagents/materials/analysis tools: EH. Wrote the paper: ADH AMC EH. Designed software: ADH.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Algorithms
Is the Subject Area "Algorithms" applicable to this article?

Thanks for your feedback.
Image analysis
Is the Subject Area "Image analysis" applicable to this article?

Thanks for your feedback.
Machine learning algorithms
Is the Subject Area "Machine learning algorithms" applicable to this article?

Thanks for your feedback.
Computer software
Is the Subject Area "Computer software" applicable to this article?

Thanks for your feedback.
Computational pipelines
Is the Subject Area "Computational pipelines" applicable to this article?

Thanks for your feedback.
Fluorescence imaging
Is the Subject Area "Fluorescence imaging" applicable to this article?

Thanks for your feedback.
Open source software
Is the Subject Area "Open source software" applicable to this article?

Thanks for your feedback.
Saccharomyces cerevisiae
Is the Subject Area "Saccharomyces cerevisiae" applicable to this article?

Thanks for your feedback.