About the Authors

Naoki Tanigawa

Affiliation Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

Toshitsugu Fujita

Affiliation Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

Hodaka Fujii

hodaka@biken.osaka-u.ac.jp

Affiliation Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

Competing Interests

The authors filed a patent application on ORNi-PCR. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials. The patent details are as follows: Name: Method for suppressing amplification of specific nucleic acid sequences; Number: Japanese Patent Application No. 2014-176018.

Author Contributions

Conceived and designed the experiments: NT TF HF. Performed the experiments: NT TF HF. Analyzed the data: NT TF HF. Contributed reagents/materials/analysis tools: NT TF HF. Contributed to the writing of the manuscript: NT HF. Initiated, directed and supervised the study: HF.