Identification and Biochemical Characterization of an Acid Sphingomyelinase-Like Protein from the Bacterial Plant Pathogen Ralstonia solanacearum that Hydrolyzes ATP to AMP but Not Sphingomyelin to Ceramide

Michael V. Airola; Jessica M. Tumolo; Justin Snider; Yusuf A. Hannun

doi:10.1371/journal.pone.0105830

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Peer-reviewed

Research Article

Identification and Biochemical Characterization of an Acid Sphingomyelinase-Like Protein from the Bacterial Plant Pathogen Ralstonia solanacearum that Hydrolyzes ATP to AMP but Not Sphingomyelin to Ceramide

Michael V. Airola,

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America
⨯
Jessica M. Tumolo,

Affiliation Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina, United States of America
⨯
Justin Snider,

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America
⨯
Yusuf A. Hannun

* E-mail: yusuf.hannun@sbumed.org

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America
⨯

Identification and Biochemical Characterization of an Acid Sphingomyelinase-Like Protein from the Bacterial Plant Pathogen Ralstonia solanacearum that Hydrolyzes ATP to AMP but Not Sphingomyelin to Ceramide

Michael V. Airola,
Jessica M. Tumolo,
Justin Snider,
Yusuf A. Hannun

Published: August 21, 2014
https://doi.org/10.1371/journal.pone.0105830

About the Authors

Michael V. Airola

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America

Jessica M. Tumolo

Affiliation Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina, United States of America

Justin Snider

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America

Yusuf A. Hannun

* E-mail: yusuf.hannun@sbumed.org

Affiliation Department of Medicine and the Stony Brook University Cancer Center, Stony Brook University, Stony Brook, New York, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MVA YAH. Performed the experiments: MVA JMT. Analyzed the data: MVA YAH. Contributed reagents/materials/analysis tools: JS. Contributed to the writing of the manuscript: MVA YAH.

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Hydrolysis
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Plant bacterial pathogens
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Nucleotides
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Bacterial biochemistry
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Lipids
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Phosphates
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Bacillus cereus
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Ralstonia
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