Reproducibility and Consistency of In Vitro Nucleosome Reconstitutions Demonstrated by Invitrosome Isolation and Sequencing

Colton E. Kempton; Justin R. Heninger; Steven M. Johnson

doi:10.1371/journal.pone.0103752

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Open Access

Peer-reviewed

Research Article

Reproducibility and Consistency of In Vitro Nucleosome Reconstitutions Demonstrated by Invitrosome Isolation and Sequencing

Colton E. Kempton,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America
⨯
Justin R. Heninger,

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America
⨯
Steven M. Johnson

* E-mail: stevenj@byu.edu

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America
⨯

Reproducibility and Consistency of In Vitro Nucleosome Reconstitutions Demonstrated by Invitrosome Isolation and Sequencing

Colton E. Kempton,
Justin R. Heninger,
Steven M. Johnson

Published: August 5, 2014
https://doi.org/10.1371/journal.pone.0103752

About the Authors

Colton E. Kempton

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America

Justin R. Heninger

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America

Steven M. Johnson

* E-mail: stevenj@byu.edu

Affiliation Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SMJ. Performed the experiments: SMJ CEK JRH. Analyzed the data: SMJ CEK. Contributed to the writing of the manuscript: SMJ CEK.

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Nucleosomes
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Plasmid construction
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DNA isolation
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Histones
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Nucleosome mapping
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Genomics
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Reproducibility
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Gene regulation
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