About the Authors

David S. Boyle

Contributed equally to this work with: David S. Boyle, Ruth McNerney

Affiliation Program for Appropriate Technology in Health, Seattle, WA, United States of America

Ruth McNerney

Contributed equally to this work with: David S. Boyle, Ruth McNerney

Affiliation Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom

Hwee Teng Low

Affiliation Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom

Brandon Troy Leader

Affiliation Program for Appropriate Technology in Health, Seattle, WA, United States of America

Ailyn C. Pérez-Osorio

Affiliation Washington State Department of Health, Public Health Laboratories, Shoreline, WA, United States of America

Jessica C. Meyer

Affiliation TwistDx Limited, Cambridge, United Kingdom

Denise M. O'Sullivan

Affiliation Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom

David G. Brooks

Affiliation TwistDx Limited, Cambridge, United Kingdom

Olaf Piepenburg

Affiliation TwistDx Limited, Cambridge, United Kingdom

Matthew S. Forrest

m.forrest@twistdx.co.uk

Affiliation TwistDx Limited, Cambridge, United Kingdom

Competing Interests

JCM, DGB, OP, and MSF are employed by TwistDx, and have a stake in the success of its technology. TwistDx is one of the funders of this study and is a UK-based company, with a commercial interest in the use of the RPA technology for the detection of hospital-acquired pathogens. TwistDx Ltd, is the developer and manufacturer of RPA technology and is part of the of the Alere group of companies. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. DSB, BTL, APO, RM, HTL, and DOS have no financial interests in this technology.

Author Contributions

Conceived and designed the experiments: DSB RM APO DGB OP MSF. Performed the experiments: DSB HTL BTL APO DMO JCM MSF. Analyzed the data: DSB RM MSF. Contributed reagents/materials/analysis tools: DSB RM APO MSF. Contributed to the writing of the manuscript: DSB RM MSF.