Two Structurally Different Dienelactone Hydrolases (TfdEI and TfdEII) from Cupriavidus necator JMP134 Plasmid pJP4 Catalyse Cis- and Trans-Dienelactones with Similar Efficiency

Ajit Kumar; Balakrishna Pillay; Ademola O. Olaniran

doi:10.1371/journal.pone.0101801

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Peer-reviewed

Research Article

Two Structurally Different Dienelactone Hydrolases (TfdEI and TfdEII) from Cupriavidus necator JMP134 Plasmid pJP4 Catalyse Cis- and Trans-Dienelactones with Similar Efficiency

Ajit Kumar,

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa
⨯
Balakrishna Pillay,

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa
⨯
Ademola O. Olaniran

* E-mail: olanirana@ukzn.ac.za

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa
⨯

Two Structurally Different Dienelactone Hydrolases (TfdEI and TfdEII) from Cupriavidus necator JMP134 Plasmid pJP4 Catalyse Cis- and Trans-Dienelactones with Similar Efficiency

Ajit Kumar,
Balakrishna Pillay,
Ademola O. Olaniran

Published: July 23, 2014
https://doi.org/10.1371/journal.pone.0101801

About the Authors

Ajit Kumar

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa

Balakrishna Pillay

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa

Ademola O. Olaniran

* E-mail: olanirana@ukzn.ac.za

Affiliation Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, Republic of South Africa

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AK. Performed the experiments: AK. Analyzed the data: AK AO. Contributed reagents/materials/analysis tools: AK AO BP. Contributed to the writing of the manuscript: AK AO BP.

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Necator
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Hydrolases
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Pseudomonas
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Enzymes
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Hydrolysis
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Enzyme purification
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Protein structure prediction
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Enzyme assays
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