A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Chuan Yu; Yaguang Zhang; Shaohua Yao; Yuquan Wei

doi:10.1371/journal.pone.0098282

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Open Access

Peer-reviewed

Research Article

A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Chuan Yu ,

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Yaguang Zhang ,

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Shaohua Yao ,

* E-mail: Shaohuayao@scu.edu.cn

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Yuquan Wei

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯

A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Chuan Yu,
Yaguang Zhang,
Shaohua Yao,
Yuquan Wei

Published: June 5, 2014
https://doi.org/10.1371/journal.pone.0098282

About the Authors

Chuan Yu

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China

Yaguang Zhang

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China

Shaohua Yao

* E-mail: Shaohuayao@scu.edu.cn

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China

Yuquan Wei

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SHY YQW. Performed the experiments: CY YGZ. Analyzed the data: CY SHY. Contributed reagents/materials/analysis tools: SHY YQW. Wrote the paper: CY SHY.

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Mutation detection
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TALENs
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Zebrafish
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Polymerase chain reaction
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Point mutation
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Deletion mutation
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Gene pool
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Genomics
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