Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion

Tien Chye Tan; Oliver Spadiut; Rosaria Gandini; Dietmar Haltrich; Christina Divne

doi:10.1371/journal.pone.0086736

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Open Access

Peer-reviewed

Research Article

Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion

Tien Chye Tan ,

Contributed equally to this work with: Tien Chye Tan, Oliver Spadiut

Affiliation Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
⨯
Oliver Spadiut ,

Contributed equally to this work with: Tien Chye Tan, Oliver Spadiut

Affiliation School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
⨯
Rosaria Gandini,

Affiliation Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
⨯
Dietmar Haltrich,

Affiliation Food Biotechnology Laboratory, BOKU University of Natural Resources and Life Sciences, Vienna, Austria
⨯
Christina Divne

* E-mail: divne@biotech.kth.se

Affiliations Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden
⨯

Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion

Tien Chye Tan,
Oliver Spadiut,
Rosaria Gandini,
Dietmar Haltrich,
Christina Divne

Published: January 21, 2014
https://doi.org/10.1371/journal.pone.0086736

About the Authors

Tien Chye Tan

Contributed equally to this work with: Tien Chye Tan, Oliver Spadiut

Affiliation Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden

Oliver Spadiut

Contributed equally to this work with: Tien Chye Tan, Oliver Spadiut

Affiliation School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden

Rosaria Gandini

Affiliation Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden

Dietmar Haltrich

Affiliation Food Biotechnology Laboratory, BOKU University of Natural Resources and Life Sciences, Vienna, Austria

Christina Divne

* E-mail: divne@biotech.kth.se

Affiliations Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TCT OS RG DH CD. Performed the experiments: TCT OS RG CD. Analyzed the data: TCT OS RG DH CD. Contributed reagents/materials/analysis tools: DH CD. Wrote the paper: TCT OS RG DH CD.

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Oxidation
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Flavin
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Glucose
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Galactose
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Binding analysis
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Crystal structure
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Hydrogen bonding
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Electron acceptors
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