About the Authors

Han-Pil Choi

Affiliation Dept of Biomedical Engineering, Boston University, Boston, Massachusetts, United States of America

Silvia Juarez

Affiliation Proteomic Facility, CNB-National Centre for Biotechnology, CSIC, Darwin 3, Madrid, Spain

Sergio Ciordia

Affiliation Proteomic Facility, CNB-National Centre for Biotechnology, CSIC, Darwin 3, Madrid, Spain

Marisol Fernandez

Affiliation Proteomic Facility, CNB-National Centre for Biotechnology, CSIC, Darwin 3, Madrid, Spain

Rafael Bargiela

Affiliation Spanish National Research Council (CSIC), Institute of Catalysis, Madrid, Spain

Juan P. Albar

Affiliation Proteomic Facility, CNB-National Centre for Biotechnology, CSIC, Darwin 3, Madrid, Spain

Varun Mazumdar

Affiliation Bioinformatics Program, Boston University, Boston, Massachusetts, United States of America

Brian P. Anton

Affiliation New England Biolabs, Ipswich, Massachusetts, United States of America

Simon Kasif

Affiliations Dept of Biomedical Engineering, Boston University, Boston, Massachusetts, United States of America, Bioinformatics Program, Boston University, Boston, Massachusetts, United States of America

Manuel Ferrer

steffen@bu.edu (MS); mferrer@icp.csic.es (MF)

Affiliation Spanish National Research Council (CSIC), Institute of Catalysis, Madrid, Spain

Martin Steffen

steffen@bu.edu (MS); mferrer@icp.csic.es (MF)

Affiliations Dept of Biomedical Engineering, Boston University, Boston, Massachusetts, United States of America, Dept of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America

Competing Interests

Author Brian P Anton is an employee of New England Biolabs. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MS M. Ferrer. Performed the experiments: HPC SJ SC MF RB JPA. Analyzed the data: HPC SJ SC MF RB JPA M. Ferrer MS VM. Wrote the paper: MS M. Ferrer. Performed the experiments on recombinant protein: HPC MS. Performed the experiments on native protein: SJ SC M. Fernandez RB JPA. Provided oversight of the informatics database used to derive the protein clusters and sequence homology for the studied proteins: SK BA. Contributed the conceptual implementation of COMBREX: SK MS.