Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

Patrik Inderbitzin; R. Michael Davis; Richard M. Bostock; Krishna V. Subbarao

doi:10.1371/journal.pone.0065990

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Open Access

Peer-reviewed

Research Article

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

Patrik Inderbitzin,

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America
⨯
R. Michael Davis,

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America
⨯
Richard M. Bostock,

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America
⨯
Krishna V. Subbarao

* E-mail: kvsubbarao@ucdavis.edu

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America
⨯

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

Patrik Inderbitzin,
R. Michael Davis,
Richard M. Bostock,
Krishna V. Subbarao

Published: June 18, 2013
https://doi.org/10.1371/journal.pone.0065990

About the Authors

Patrik Inderbitzin

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America

R. Michael Davis

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America

Richard M. Bostock

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America

Krishna V. Subbarao

* E-mail: kvsubbarao@ucdavis.edu

Affiliation Department of Plant Pathology, University of California Davis, Davis, California, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: PI RMD RMB KVS. Performed the experiments: PI. Analyzed the data: PI. Contributed reagents/materials/analysis tools: PI RMD RMB KVS. Wrote the paper: PI RMD RMB KVS.

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Polymerase chain reaction
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Plant fungal pathogens
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Verticillium
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Sequence alignment
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Genetic loci
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Gel electrophoresis
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Phylogenetic analysis
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DNA annealing
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