About the Authors

Jan Leo Rinnenthal

Contributed equally to this work with: Jan Leo Rinnenthal, Christian Börnchen

Affiliations German Rheumatism Research Center, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Christian Börnchen

Contributed equally to this work with: Jan Leo Rinnenthal, Christian Börnchen

Affiliation Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

Helena Radbruch

Affiliations Max-Delbrück Center for Molecular Medicine, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Volker Andresen

Affiliation LaVision Biotec GmbH, Bielefeld, Germany

Agata Mossakowski

Affiliations German Rheumatism Research Center, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Volker Siffrin

Affiliations Max-Delbrück Center for Molecular Medicine, Berlin, Germany, Neurology Department, Johannes Gutenberg University Mainz, Mainz, Germany

Thomas Seelemann

Affiliation LaVision, Göttingen, Germany

Heinrich Spiecker

Affiliation LaVision Biotec GmbH, Bielefeld, Germany

Ingrid Moll

Affiliation Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

Josephine Herz

Current address: Neurology Department, University Duisburg-Essen, Essen, Germany

Affiliations Max-Delbrück Center for Molecular Medicine, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Anja E. Hauser

Affiliations German Rheumatism Research Center, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Frauke Zipp

Affiliations Max-Delbrück Center for Molecular Medicine, Berlin, Germany, Neurology Department, Johannes Gutenberg University Mainz, Mainz, Germany

Martin J. Behne

These authors also contributed equally to this work.

Affiliation Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

Raluca Niesner

niesner@drfz.de

These authors also contributed equally to this work.

Affiliations German Rheumatism Research Center, Berlin, Germany, Max-Delbrück Center for Molecular Medicine, Berlin, Germany, Charité – University of Medicine, Berlin, Germany

Competing Interests

VA and HS are with LaVision BioTec, Bielefeld, Germany and TS is with LaVision, Göttingen, Germany. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: J-LR CB RN. Performed the experiments: J-LR CB RN. Analyzed the data: J-LR CB RN. Contributed reagents/materials/analysis tools: J-LR RN TS. Wrote the paper: J-LR VA MB RN. Edited the manuscript: HR VS FZ. Initiated and supervised the project: RN VA MB. Provided expertise in mouse handling and brain slice preperation: HR AEH. Performed experiments and provided expertise in EAE: HR AM JH VS FZ. Gave technical expertise for FLIM by time-gated field detection and parallelized TCSPC point detection: VA TS HS. Developed evaluation software RINIFLIM: J-LR.