About the Authors
- Erlinda Fernández
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Contributed equally to this work with: Erlinda Fernández, Jorge R. Toledo
Affiliation Division of Animal Biotechnology, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Jorge R. Toledo
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Contributed equally to this work with: Erlinda Fernández, Jorge R. Toledo
Affiliation Department of Physiopathology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile
- Lídice Méndez
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Affiliation Division of Animal Biotechnology, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Nemecio González
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Affiliation Technological Development Department, Center for Genetic Engineering and Biotechnology of Camaguey, Camaguey, Cuba
- Francisco Parra
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Affiliation Department of Biochemistry and Molecular Biology, Instituto Universitario de Biotecnología de Asturias, University of Oviedo, Oviedo, Spain
- José M. Martín-Alonso
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Affiliation Department of Biochemistry and Molecular Biology, Instituto Universitario de Biotecnología de Asturias, University of Oviedo, Oviedo, Spain
- Miladys Limonta
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Affiliation Division of Technological Development, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Kosara Sánchez
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Affiliation Division of Development of Formulations, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Ania Cabrales
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Affiliation Peptide Synthesis Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Mario P. Estrada
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Affiliation Division of Animal Biotechnology, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Alina Rodríguez-Mallón
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Affiliation Division of Animal Biotechnology, Center for Genetic Engineering and Biotechnology, Havana, Cuba
- Omar Farnós
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* E-mail: omar.farnos@yahoo.com
Affiliation Division of Animal Biotechnology, Center for Genetic Engineering and Biotechnology, Havana, Cuba
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
Designed the experiments, participated directly in assessments for thermal and conformational stability, in experimentation with animals, in the analysis of the results and drafted the manuscript: OF EF JRT. Designed and conducted the experiments related to RHDV VLPs conjugates and the evaluation of immune responses to CSFV: LM. Designed and carried out the process scale-up, the production of active raw material starting from 10-L bioreactions, and participated in formulation and filling at major scale: NG. Participated in the design of the experiments and in the assessments for protective efficacy: FP JMMA. Carried out peptide synthesis and conjugation: AC. Participated in the process scale-up, in quality control assessments and in formulation related issues: ML KS. Participated in the design of the experiments and in the analysis of the results: MPE ARM. Read and approved the manuscript: EF JRT LM NG FP JMMA ML KS AC MPE ARM OF. Conceived and designed the experiments: OF EF JRT LM NG FP JMMA MPE ARM. Performed the experiments: OF EF JRT LM NG FP JMMA ML KS. Analyzed the data: OF EF JRT MPE ARM. Contributed reagents/materials/analysis tools: AC. Wrote the paper: OF EF JRT.