Validation of Reference Genes for Real-Time PCR of Reproductive System in the Black Tiger Shrimp

Rungnapa Leelatanawit; Amornpan Klanchui; Umaporn Uawisetwathana; Nitsara Karoonuthaisiri

doi:10.1371/journal.pone.0052677

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Open Access

Peer-reviewed

Research Article

Validation of Reference Genes for Real-Time PCR of Reproductive System in the Black Tiger Shrimp

Rungnapa Leelatanawit,

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand
⨯
Amornpan Klanchui,

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand
⨯
Umaporn Uawisetwathana,

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand
⨯
Nitsara Karoonuthaisiri

* E-mail: nitsara.kar@biotec.or.th

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand
⨯

Validation of Reference Genes for Real-Time PCR of Reproductive System in the Black Tiger Shrimp

Rungnapa Leelatanawit,
Amornpan Klanchui,
Umaporn Uawisetwathana,
Nitsara Karoonuthaisiri

Published: December 28, 2012
https://doi.org/10.1371/journal.pone.0052677

About the Authors

Rungnapa Leelatanawit

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand

Amornpan Klanchui

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand

Umaporn Uawisetwathana

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand

Nitsara Karoonuthaisiri

* E-mail: nitsara.kar@biotec.or.th

Affiliation Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: NK RL. Performed the experiments: RL AK UU. Analyzed the data: RL AK UU. Contributed reagents/materials/analysis tools: SK. Wrote the paper: NK RL AK UU.

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Shrimp
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Domestic animals
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Gene expression
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Ovaries
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Ribosomal RNA
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Polymerase chain reaction
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Reproductive system analysis
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Reproductive system
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