About the Authors

Tatsuhiko Ozawa

Contributed equally to this work with: Tatsuhiko Ozawa, Xiuhong Piao

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Xiuhong Piao

Contributed equally to this work with: Tatsuhiko Ozawa, Xiuhong Piao

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Eiji Kobayashi

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Yue Zhou

Affiliation Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Hiroaki Sakurai

Affiliation Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Tsugunobu Andoh

Affiliation Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Aishun Jin

Affiliations Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan, Department of Immunology, College of Basic Medical Sciences, Harbin Medical University, Nangang District, Harbin, China

Hiroyuki Kishi

immkishi@med.u-toyama.ac.jp

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Atsushi Muraguchi

Affiliation Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

Competing Interests

AM was supported by a research fund from Vivalis (Nantes, France.) The ISAAC method is covered by patents that have been exclusively licensed to Vivalis (Nantes, France.) Patent details: Pub.No.: WO/2009/017226, International Application No.: PCT/JP2008/063874, Title: CELL SCREENING METHOD; HK is one of Scientific Advisory Board Members of Vivalis. Microwell array chips are now provided to us by Vivalis. There are no further patents, products in development or marketed products to declare. This does not alter the authors′ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: TO EK HS AJ HK AM. Performed the experiments: TO XP YZ TA. Analyzed the data: TO XP HS HK AM. Wrote the paper: TO HK AM.