About the Authors

Louise Knudsen

lsek@novonordisk.com

Affiliations Receptor Systems Biology Laboratory, Hagedorn Research Institute, Gentofte, Denmark, Department of Insulin and Incretin Biology, Hagedorn Research Institute, Gentofte, Denmark, Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark

Bo Falck Hansen

Affiliation Insulin Biology, Novo Nordisk A/S, Måløv, Denmark

Pia Jensen

Affiliation Insulin Biology, Novo Nordisk A/S, Måløv, Denmark

Thomas Åskov Pedersen

Affiliation Insulin Biology, Novo Nordisk A/S, Måløv, Denmark

Kirsten Vestergaard

Affiliation Insulin Biology, Novo Nordisk A/S, Måløv, Denmark

Lauge Schäffer

Affiliation Department of Insulin and Incretin Biology, Hagedorn Research Institute, Gentofte, Denmark

Blagoy Blagoev

Affiliation Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark

Martin B. Oleksiewicz

Affiliation Arsanis Biosciences, Vienna, Austria

Vladislav V. Kiselyov

Affiliations Receptor Systems Biology Laboratory, Hagedorn Research Institute, Gentofte, Denmark, Department of Insulin and Incretin Biology, Hagedorn Research Institute, Gentofte, Denmark

Pierre De Meyts

Affiliations Receptor Systems Biology Laboratory, Hagedorn Research Institute, Gentofte, Denmark, Department of Insulin and Incretin Biology, Hagedorn Research Institute, Gentofte, Denmark

Competing Interests

The authors have read the journal’s policy and have the following conflicts: Thomas Åskov Pedersen, Kirsten Vestergaard, Pierre De Meyts, Vladislav V. Kiselyov, Bo Falck Hansen, Pia Jensen, and Lauge Schäffer were employees of Novo Nordisk A/S. Pierre De Meyts, Bo Falck Hansen, Pia Jensen, Lauge Schäffer Louise Knudsen, Kirsten Vestergaard and Thomas Åskov Pedersen own Novo Nordisk stocks. Novo Nordisk were a funder for this study and provided the S661 and S961 for the study. Martin B. Oleksiewicz was employed by Arsanis Biosciences at the time of the study. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: LK PDM MBO LS. Performed the experiments: LK BFH PJ TÅP KV. Analyzed the data: LK BFH LS PDM MBO TÅP VVK. Contributed reagents/materials/analysis tools: LS BB. Wrote the paper: LK MBO PDM VVK. Discussing of data and proofreading of the manuscript: LK BFH PJ TÅP KV LS BB MBO VVK PDM.