Profiling Nematode Communities in Unmanaged Flowerbed and Agricultural Field Soils in Japan by DNA Barcode Sequencing

Hisashi Morise; Erika Miyazaki; Shoko Yoshimitsu; Toshihiko Eki

doi:10.1371/journal.pone.0051785

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Open Access

Peer-reviewed

Research Article

Profiling Nematode Communities in Unmanaged Flowerbed and Agricultural Field Soils in Japan by DNA Barcode Sequencing

Hisashi Morise,

Affiliation Molecular Genetics Laboratory, Division of Bioscience and Biotechnology, Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan
⨯
Erika Miyazaki,

Affiliation Molecular Genetics Laboratory, Division of Bioscience and Biotechnology, Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan
⨯
Shoko Yoshimitsu,

Affiliation Molecular Genetics Laboratory, Division of Bioscience and Biotechnology, Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan
⨯
Toshihiko Eki

* E-mail: eki@ens.tut.ac.jp

Affiliation Molecular Genetics Laboratory, Division of Bioscience and Biotechnology, Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan
⨯

Profiling Nematode Communities in Unmanaged Flowerbed and Agricultural Field Soils in Japan by DNA Barcode Sequencing

Hisashi Morise,
Erika Miyazaki,
Shoko Yoshimitsu,
Toshihiko Eki

Published: December 20, 2012
https://doi.org/10.1371/journal.pone.0051785

About the Authors

Hisashi Morise

Affiliation Molecular Genetics Laboratory, Division of Bioscience and Biotechnology, Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan

Erika Miyazaki

Shoko Yoshimitsu

Toshihiko Eki

* E-mail: eki@ens.tut.ac.jp

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: HM TE. Performed the experiments: HM EM SY. Analyzed the data: HM TE. Contributed reagents/materials/analysis tools: HM EM. Wrote the paper: TE.

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Agricultural soil science
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DNA barcoding
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Nematoda
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Polymerase chain reaction
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Phylogenetic analysis
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Taxonomy
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DNA
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Flowering plants
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