Direct Chromatin PCR (DC-PCR): Hypotonic Conditions Allow Differentiation of Chromatin States during Thermal Cycling

Sergei Vatolin; Shahper N. Khan; Frederic J. Reu

doi:10.1371/journal.pone.0044690

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Open Access

Peer-reviewed

Research Article

Direct Chromatin PCR (DC-PCR): Hypotonic Conditions Allow Differentiation of Chromatin States during Thermal Cycling

Sergei Vatolin,

Affiliation Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America
⨯
Shahper N. Khan,

Affiliation Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America
⨯
Frederic J. Reu

* E-mail: reuf@ccf.org

Affiliations Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America, Department of Hematologic Oncology and Blood Disorders, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America
⨯

Direct Chromatin PCR (DC-PCR): Hypotonic Conditions Allow Differentiation of Chromatin States during Thermal Cycling

Sergei Vatolin,
Shahper N. Khan,
Frederic J. Reu

Published: September 12, 2012
https://doi.org/10.1371/journal.pone.0044690

About the Authors

Sergei Vatolin

Affiliation Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America

Shahper N. Khan

Affiliation Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America

Frederic J. Reu

* E-mail: reuf@ccf.org

Affiliations Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America, Department of Hematologic Oncology and Blood Disorders, Taussig Cancer Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SV FJR. Performed the experiments: SV SNK. Analyzed the data: SV SNK FJR. Contributed reagents/materials/analysis tools: FJR. Wrote the paper: SV FJR.

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Chromatin
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Polymerase chain reaction
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Drug screening
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DNA transcription
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Protease inhibitor therapy
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Small molecules
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Epigenetics
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Myeloma cells
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