About the Authors
- Shiva Kumar
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Contributed equally to this work with: Shiva Kumar, Suguna Badireddy
Current address: Department of Chemistry, University of Washington, Seattle, Washington, United States of America
Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore
- Suguna Badireddy
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Contributed equally to this work with: Shiva Kumar, Suguna Badireddy
Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore
- Kuntal Pal
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Current address: Van Andel Institute, Grand Rapids, Michigan, United States of America
Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore
- Shikha Sharma
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Affiliation Institute of Microbial Technology, CSIR, Sector 39-A, Chandigarh, India
- Chandni Arora
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Affiliation Institute of Microbial Technology, CSIR, Sector 39-A, Chandigarh, India
- Saurabh K. Garg
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Current address: Vollum Institute, Oregon Health and Science University, Portland, Oregon, United States of America
Affiliation Institute of Microbial Technology, CSIR, Sector 39-A, Chandigarh, India
- Mohamed Suhail Alam
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Current address: University of Notre Dame, Notre Dame, Indiana, United States of America
Affiliation Institute of Microbial Technology, CSIR, Sector 39-A, Chandigarh, India
- Pushpa Agrawal
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* E-mail: pushpa@imtech.res.in (PA); dbsgsa@nus.edu.sg (GSA); dbsks@nus.edu.sg (KS)
Affiliation Institute of Microbial Technology, CSIR, Sector 39-A, Chandigarh, India
- Ganesh Srinivasan Anand
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* E-mail: pushpa@imtech.res.in (PA); dbsgsa@nus.edu.sg (GSA); dbsks@nus.edu.sg (KS)
Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore
- Kunchithapadam Swaminathan
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* E-mail: pushpa@imtech.res.in (PA); dbsgsa@nus.edu.sg (GSA); dbsks@nus.edu.sg (KS)
Affiliation Department of Biological Sciences, National University of Singapore, Singapore, Singapore
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
Wrote the paper: SB SK PA. Initiated the project and designed biophysical studies: PA. Proposed and designed the hydrogen deuterium exchange studies: GSA KS. Prepared all overexpression constructs, prepared proteins and performed biophysical studies: SS CA SKG MSA. Expressed, purified the proteins and involved in Hydrogen-deutrium (HDX) studies: SK KP. Performed HDX data collection and interpretation: GSA SB SK. Analyzed the biophysical data: PA. Analyzed the HDX data: SK SB GSA KS. Provided the overexpression constructs and contributed all material needed for the biophysical studies: PA. Provided all material for protein overexpression, purification and HDX studies: KS. Provided all material needed for the HDX studies: GSA. Corrected the manuscript: SK PA GSA KS.