About the Authors

Masayuki Takahashi

Contributed equally to this work with: Masayuki Takahashi, Reyad A. Elbarbary

Affiliation Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

Reyad A. Elbarbary

Contributed equally to this work with: Masayuki Takahashi, Reyad A. Elbarbary

Affiliation Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

Mayumi Abe

Affiliation Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

Mari Sato

Affiliation Department of Biochemistry and Molecular Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

Tetsuo Yoshida

Affiliation Biologics Research Laboratories, Kyowa Hakko Kirin Company Limited, Machida, Japan

Yoji Yamada

Affiliation Biologics Research Laboratories, Kyowa Hakko Kirin Company Limited, Machida, Japan

Masato Tamura

Affiliation Department of Biochemistry and Molecular Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

Masayuki Nashimoto

mnashimoto@nupals.ac.jp

Affiliation Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

Competing Interests

The authors have read the journal’s policy and have the following conflicts: The authors TY and YY are employees of Kyowa Hakko Kirin. Niigata University of Pharmacy and Applied Life Sciences has a pending patent application on the miRNA-targeting sgRNAs. The authors TY, YY and MN are named as inventors in this application. This does not alter the authors’ adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: RAE MN. Performed the experiments: M. Takahashi RAE MA MS M. Tamura. Analyzed the data: M. Takahashi RAE TY YY M. Tamura MN. Wrote the paper: MN.