Imaging Immune and Metabolic Cells of Visceral Adipose Tissues with Multimodal Nonlinear Optical Microscopy

Yasuyo Urasaki; Mary G. Johlfs; Ronald R. Fiscus; Thuc T. Le

doi:10.1371/journal.pone.0038418

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Open Access

Peer-reviewed

Research Article

Imaging Immune and Metabolic Cells of Visceral Adipose Tissues with Multimodal Nonlinear Optical Microscopy

Yasuyo Urasaki,

Affiliations Human Health and Environment Program, Desert Research Institute, Las Vegas, Nevada, United States of America, Department of Drug Development, Nevada Cancer Institute, Las Vegas, Nevada, United States of America
⨯
Mary G. Johlfs,

Affiliation Center for Diabetes and Obesity Prevention, Treatment, Research and Education, and the College of Pharmacy, Roseman University of Health Sciences, Henderson, Nevada, United States of America
⨯
Ronald R. Fiscus,

Affiliation Center for Diabetes and Obesity Prevention, Treatment, Research and Education, and the College of Pharmacy, Roseman University of Health Sciences, Henderson, Nevada, United States of America
⨯
Thuc T. Le

* E-mail: thuc.le@dri.edu

Affiliations Human Health and Environment Program, Desert Research Institute, Las Vegas, Nevada, United States of America, Department of Drug Development, Nevada Cancer Institute, Las Vegas, Nevada, United States of America
⨯

Imaging Immune and Metabolic Cells of Visceral Adipose Tissues with Multimodal Nonlinear Optical Microscopy

Yasuyo Urasaki,
Mary G. Johlfs,
Ronald R. Fiscus,
Thuc T. Le

Published: June 11, 2012
https://doi.org/10.1371/journal.pone.0038418

About the Authors

Yasuyo Urasaki

Affiliations Human Health and Environment Program, Desert Research Institute, Las Vegas, Nevada, United States of America, Department of Drug Development, Nevada Cancer Institute, Las Vegas, Nevada, United States of America

Mary G. Johlfs

Affiliation Center for Diabetes and Obesity Prevention, Treatment, Research and Education, and the College of Pharmacy, Roseman University of Health Sciences, Henderson, Nevada, United States of America

Ronald R. Fiscus

Thuc T. Le

* E-mail: thuc.le@dri.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TTL YU. Performed the experiments: TTL YU. Analyzed the data: TTL YU. Contributed reagents/materials/analysis tools: TTL YU RRF MGJ. Wrote the paper: TTL.

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Lipids
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Macrophages
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Obesity
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Adipocytes
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Fatty acids
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Fluorescence imaging
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Adipose tissue
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Cell staining
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