Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

Nao Nishida; Yoriko Mawatari; Megumi Sageshima; Katsushi Tokunaga

doi:10.1371/journal.pone.0029967

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Open Access

Peer-reviewed

Research Article

Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

Nao Nishida ,

* E-mail: nishida-75@umin.ac.jp

Affiliations Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan, Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Japan
⨯
Yoriko Mawatari,

Affiliations Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan, Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Japan
⨯
Megumi Sageshima,

Affiliation Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
⨯
Katsushi Tokunaga

Affiliation Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
⨯

Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

Nao Nishida,
Yoriko Mawatari,
Megumi Sageshima,
Katsushi Tokunaga

Published: January 13, 2012
https://doi.org/10.1371/journal.pone.0029967

About the Authors

Nao Nishida

* E-mail: nishida-75@umin.ac.jp

Affiliations Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan, Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Japan

Yoriko Mawatari

Megumi Sageshima

Affiliation Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

Katsushi Tokunaga

Affiliation Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: NN KT. Performed the experiments: YM MS. Analyzed the data: NN YM MS. Contributed reagents/materials/analysis tools: NN YM MS. Wrote the paper: NN KT.

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Polymerase chain reaction
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Single nucleotide polymorphisms
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Genotyping
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DNA polymerase
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Variant genotypes
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Polymerases
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Genome-wide association studies
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Genomics
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