Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

Pamela D. Schoppee Bortz; Brian R. Wamhoff

doi:10.1371/journal.pone.0026015

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Peer-reviewed

Research Article

Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

Pamela D. Schoppee Bortz ,

* E-mail: pamela.schoppee.bortz@virginia.edu (PDSB); wamhoff@virginia.edu (BRW)

Affiliation Cardiovascular Division, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America
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Brian R. Wamhoff

* E-mail: pamela.schoppee.bortz@virginia.edu (PDSB); wamhoff@virginia.edu (BRW)

Affiliations Cardiovascular Division, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America, Robert M. Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, Virginia, United States of America
⨯

Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

Pamela D. Schoppee Bortz,
Brian R. Wamhoff

Published: October 25, 2011
https://doi.org/10.1371/journal.pone.0026015

About the Authors

Pamela D. Schoppee Bortz

* E-mail: pamela.schoppee.bortz@virginia.edu (PDSB); wamhoff@virginia.edu (BRW)

Affiliation Cardiovascular Division, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America

Brian R. Wamhoff

* E-mail: pamela.schoppee.bortz@virginia.edu (PDSB); wamhoff@virginia.edu (BRW)

Affiliations Cardiovascular Division, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America, Robert M. Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, Virginia, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: PDSB BRW. Performed the experiments: PDSB. Analyzed the data: PDSB. Contributed reagents/materials/analysis tools: PDSB. Wrote the paper: PDSB. Edited the manuscript: BRW.

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Sonication
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Chromatin immunoprecipitation
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DNA shearing
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Chromatin
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DNA extraction
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DNA electrophoresis
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Centrifugation
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Polymerase chain reaction
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