Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein

Benjamin Dälken; Robert A. Jabulowsky; Pranav Oberoi; Itai Benhar; Winfried S. Wels

doi:10.1371/journal.pone.0014404

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Peer-reviewed

Research Article

Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein

Benjamin Dälken,

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany
⨯
Robert A. Jabulowsky,

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany
⨯
Pranav Oberoi,

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany
⨯
Itai Benhar,

Affiliation Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Israel
⨯
Winfried S. Wels

* E-mail: wels@em.uni-frankfurt.de

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany
⨯

Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein

Benjamin Dälken,
Robert A. Jabulowsky,
Pranav Oberoi,
Itai Benhar,
Winfried S. Wels

Published: December 22, 2010
https://doi.org/10.1371/journal.pone.0014404

About the Authors

Benjamin Dälken

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany

Robert A. Jabulowsky

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany

Pranav Oberoi

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany

Itai Benhar

Affiliation Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Israel

Winfried S. Wels

* E-mail: wels@em.uni-frankfurt.de

Affiliation Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt am Main, Germany

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BD RAJ PO WW. Performed the experiments: BD RAJ PO. Analyzed the data: BD RAJ PO WW. Contributed reagents/materials/analysis tools: IB. Wrote the paper: BD IB WW.

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Pichia pastoris
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Yeast
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Glutathione chromatography
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Cloning
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Recombinant proteins
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Protein expression
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Cell fusion
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Protein domains
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