Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity

Manuel A. F. V. Gonçalves; Josephine M. Janssen; Maarten Holkers; Antoine A. F. de Vries

doi:10.1371/journal.pone.0010954

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Open Access

Peer-reviewed

Research Article

Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity

Manuel A. F. V. Gonçalves ,

* E-mail: m.goncalves@lumc.nl

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Josephine M. Janssen,

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Maarten Holkers,

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
⨯
Antoine A. F. de Vries

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
⨯

Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity

Manuel A. F. V. Gonçalves,
Josephine M. Janssen,
Maarten Holkers,
Antoine A. F. de Vries

Published: June 3, 2010
https://doi.org/10.1371/journal.pone.0010954

About the Authors

Manuel A. F. V. Gonçalves

* E-mail: m.goncalves@lumc.nl

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Josephine M. Janssen

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Maarten Holkers

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Antoine A. F. de Vries

Affiliation Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MAFVG AAFdV. Performed the experiments: MAFVG JMJ. Analyzed the data: MAFVG JMJ MH AAFdV. Contributed reagents/materials/analysis tools: MAFVG JMJ MH AAFdV. Wrote the paper: MAFVG. Edited the paper: AAFdV.

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Cell fusion
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Myoblasts
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Lentivirus
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Cell differentiation
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Gene expression
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Reporter genes
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Muscle differentiation
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Luciferase
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