About the Authors

Miguel Aste-Amézaga

jose_asteamezaga@merck.com

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Ningyan Zhang

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Janet E. Lineberger

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Beth A. Arnold

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Timothy J. Toner

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Mingcheng Gu

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Lingyi Huang

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Salvatore Vitelli

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Kim T. Vo

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Peter Haytko

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Jing Zhang Zhao

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Frederic Baleydier

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Sarah L'Heureux

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Hongfang Wang

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Wendy R. Gordon

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Elizabeth Thoryk

Affiliation Department of Vaccines, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Marie Blanke Andrawes

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Kittichoat Tiyanont

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Kimberly Stegmaier

Affiliations Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts, United States of America, Cancer Program, Broad Institute, Cambridge, Massachusetts, United States of America

Giovanni Roti

Affiliation Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts, United States of America

Kenneth N. Ross

Affiliation Cancer Program, Broad Institute, Cambridge, Massachusetts, United States of America

Laura L. Franlin

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Hui Wang

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Fubao Wang

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Michael Chastain

Affiliation Department of Molecular Profiling and Pharmacology, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Andrew J. Bett

Affiliation Department of Vaccines, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Laurent P. Audoly

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Jon C. Aster

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Stephen C. Blacklow

Affiliation Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, United States of America

Hans E. Huber

Affiliation Department of Biologics Research, Merck Research Laboratories, West Point, Pennsylvania, United States of America

Competing Interests

MAA, FW, NZ, AJB, JCA, and SCB are co-inventors of a patent application related to the antibodies reported in the study. The patent is entitled: “Generation and Characterization of anti-Notch Monoclonal Antibodies” (Application Serial No 61/199,753, filed on 11/20/2008). MAA, NZ, JEL, BAA, TJT, MG, LH, SV, KTV, PH, JZZ, ET, KTV, LLF, HW, FW, MC, AJB, LPA, and HEH are employees of Merck & Co., Inc. None of the authors from Merck & Co., Inc. received funding from any of the organizations (National Institutes of Health, Leukemia and Lymphoma Society, and the William Lawrence and Blanche Hughes Foundation) listed in the Financial Disclosure.

Author Contributions

Conceived and designed the experiments: MAA NZ JEL BAA SV WRG MBA KT KS GR HW FW MC AJB JCA SCB HEH. Performed the experiments: MAA JEL BAA TJT MG LH SV PH JZZ FB SL HW MBA KT GR LLF HW. Analyzed the data: MAA NZ JEL BAA SV KT KNR HW FW AJB JCA SCB HEH. Contributed reagents/materials/analysis tools: NZ BAA TJT LH SV KTV PH JZZ SL WRG ET MBA FW JCA SCB. Wrote the paper: MAA BAA KS JCA SCB HEH. Edited and approved final manuscript: MAA FW LPA JCA SCB HEH.