Fig 1.
Map of the three sites in the upper Florida Keys in Florida.
Indicating the location of the three inshore genotypes (UKI1 = Upper Keys Inshore 1 and UKI2 = Upper Keys Inshore 2) and offshore site were all four offshore genotypes that were sampled (UKO2 = Upper Keys Offshore 2). This map was constructed in ArcGIS using Florida’s Unified Reef Map from the Florida Fish and Wildlife Conservation Commission. The base layer and terms of use can be found here: https://myfwc.com/research/gis/fisheries/unified-reef-map/.
Table 1.
Coral samples collected for the RNAseq.
Fig 2.
Proportion of B. minutum and D. trenchii in the O. faveolata inshore (In1, In2, In3) and offshore (O1, O2, O4, O5) genotypes per condition after (a and c) five days and (b and d) 31 days of treatment. Bars indicate the standard error, except for offshore samples since there is one sample per genotype.
Table 2.
Transcriptome assembly statistics.
Fig 3.
Response of the symbiont and host to heat stress after five and 31 days of treatment (33°C = five days, 32°C = 31 days).
Differential gene expression analysis (DEGs) in heat stress treatment versus the control in: (a) B. minutum after five days, (b) B. minutum after 31 days, (c) D. trenchii after five days, (d) D. trenchii after 31 days, (e) coral host after five days, and (f) coral host after 31 days.
Fig 4.
Hierarchical clustering dendrogram of module-trait associations for the host transcriptome.
Each row corresponds to a module and the columns to a trait. Red are positive and blue are negative correlations with values corresponding to a significant Pearson’s correlations (P < 0.05). Traits were input as follows: Fv/Fm score that was divided in quantiles groups (6 = higher yield to 1 = lower yield), time (1 = 5 days to 4 = 31 days), treatment (0 = control, 1 = treatment), inshore genotypes (In1, In2, In3; 1 = present, 0 = absent) and grouped offshore genotypes (1 = present, 0 = absent), and D. trenchii proportion values.
Fig 5.
Boxplots of the module eigengene expression (y-axis) with respect to the genotypes (x-axis) and condition for the host.
(a) module 2 (N = 2,241), (b) Hsp70 (N = 6, p-value = 6.90 e-11, cor = -0.82) in module 2, (c) proteasome activator complex (PSME, N = 11, p-value = 9.79 e-09, cor = 0.76) in module 2, (d) module 14 (N = 468), (f) module 30 (N = 123), and (e) 40S and 60S ribosomal proteins (N = 52; p-value = 2.09 e-2, cor = 0.36) in module 15.