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cDNA synthesis  
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Evaluation of suitable reference genes for qPCR normalisation of gene expression in a Achilles tendon injury model

Neil Marr, Richard Meeson, Richard J. Piercy, John C. W. Hildyard, Chavaunne T. Thorpe

Research Article | published 27 Aug 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0306678

Views: 572Citations: 0Saves: 0 • Shares: 0

Single-cell RNA sequencing of nc886, a non-coding RNA transcribed by RNA polymerase III, with a primer spike-in strategy

Gyeong-Jin Shin, Byung-Han Choi, Hye Hyeon Eum, Areum Jo, Nayoung Kim, Huiram Kang, Dongwan Hong, Jiyoung Joan Jang, Hwi-Ho Lee, Yeon-Su Lee, Yong Sun Lee, Hae-Ock Lee

Research Article | published 27 Aug 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0301562

Views: 577Citations: 0Saves: 0 • Shares: 0

Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions

Li He, Jin Yi Wang, Qiang Jun Su, Zhao He Chen, Fang Xie

Research Article | published 06 Feb 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0287882

Views: 713Citations: 2Saves: 0 • Shares: 0

Towards high-throughput parallel imaging and single-cell transcriptomics of microbial eukaryotic plankton

Vesna Grujčić, Sami Saarenpää, John Sundh, Bengt Sennblad, Benjamin Norgren, Meike Latz, Stefania Giacomello, Rachel A. Foster, Anders F. Andersson

Research Article | published 19 Jan 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0296672

Views: 1216Citations: 4Saves: 12 • Shares: 3

In-silico prediction of RT-qPCR-high resolution melting for broad detection of emaraviruses

Alejandro Olmedo-Velarde, Francisco M. Ochoa-Corona, Adriana E. Larrea-Sarmiento, Toufic Elbeaino, Francisco Flores

Research Article | published 08 May 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0272980

Views: 1456Citations: 2Saves: 34 • Shares: 6

RC-4BC cells express nicotinic and muscarinic acetylcholine receptors

Gulsamal Zhubanova, Olena Filchakova

Research Article | published 16 Dec 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0279284

Views: 899Citations: 0Saves: 0 • Shares: 0

A prophylactic subcutaneous dose of the anticoagulant tinzaparin does not influence qPCR-based assessment of circulating levels of miRNA in humans

Abraham Nilsson, Anna Maria Nerhall, Ivan Vechetti, Lotta Fornander, Simon Wiklund, Björn Alkner, Jörg Schilcher, Ferdinand von Walden

Research Article | published 03 Nov 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0277008

Views: 908Citations: 0Saves: 3 • Shares: 2

Dysregulation of circulating miRNAs promotes the pathogenesis of diabetes-induced cardiomyopathy

Uzair Ahmed, Usman Ali Ashfaq, Muhammad Qasim, Imtiaz Ahmad, Hafiz Usman Ahmad, Muhammad Tariq, Muhammad Shareef Masoud, Saba Khaliq

Research Article | published 28 Apr 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0250773

Views: 2381Citations: 15Saves: 29 • Shares: 0

Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa

Nathaly Maldonado-Taipe, Dilan S. R. Patirange, Sandra M. Schmöckel, Christian Jung, Nazgol Emrani

Research Article | published 11 Mar 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0233821

Views: 1998Citations: 10Saves: 25 • Shares: 1

Selection of internal references for RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell lines

Yi-Hui Gu, Xi-Wei Cui, Jie-Yi Ren, Man-Mei Long, Wei Wang, Cheng-Jiang Wei, Rehanguli Aimaier, Yue-Hua Li, Man-Hon Chung, Bin Gu, Qing-Feng Li, Zhi-Chao Wang

Research Article | published 25 Feb 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0241821

Views: 1989Citations: 7Saves: 0 • Shares: 0

Reference genes for gene expression analysis in the fungal pathogen Neonectria ditissima and their use demonstrating expression up-regulation of candidate virulence genes

Liz M. Florez, Reiny W. A. Scheper, Brent M. Fisher, Paul W. Sutherland, Matthew D. Templeton, Joanna K. Bowen

Research Article | published 13 Nov 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0238157

Views: 2095Citations: 4Saves: 13 • Shares: 0

Translesion synthesis by AMV, HIV, and MMLVreverse transcriptases using RNA templates containing inosine, guanosine, and their 8-oxo-7,8-dihydropurine derivatives

Madeline M. Glennon, Austin Skinner, Mara Krutsinger, Marino J. E. Resendiz

Research Article | published 28 Aug 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0235102

Views: 1835Citations: 2Saves: 11 • Shares: 1

Selection and validation of reference genes for gene expression studies in Pseudomonas brassicacearum GS20 using real-time quantitative reverse transcription PCR

Bianxia Bai, Jiahong Ren, Fenling Bai, Lin Hao

Research Article | published 27 Jan 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0227927

Views: 5025Citations: 28Saves: 44 • Shares: 1

NaV1.6 and NaV1.7 channels are major endogenous voltage-gated sodium channels in ND7/23 cells

Jisoo Lee, Shinae Kim, Hye-mi Kim, Hyun Jeong Kim, Frank H. Yu

Research Article | published 16 Aug 2019 PLOS ONE

https://doi.org/10.1371/journal.pone.0221156

Views: 4671Citations: 14Saves: 0 • Shares: 0

Diagnostic performance of the molecular BCR-ABL1 monitoring system may impact on inclusion of CML patients in stopping trials

Birgit Spiess, Sébastien Rinaldetti, Nicole Naumann, Norbert Galuschek, Ute Kossak-Roth, Patrick Wuchter, Irina Tarnopolscaia, Diana Rose, Astghik Voskanyan, Alice Fabarius, Wolf-Karsten Hofmann, Susanne Saußele, Wolfgang Seifarth

Research Article | published 21 Mar 2019 PLOS ONE

https://doi.org/10.1371/journal.pone.0214305

Views: 4712Citations: 19Saves: 0 • Shares: 0

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