Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez; Patricia Molina-Espeja; Eva Garcia-Ruiz; Miguel Alcalde

doi:10.1371/journal.pone.0090919

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Open Access

Peer-reviewed

Research Article

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez,

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
⨯
Patricia Molina-Espeja,

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
⨯
Eva Garcia-Ruiz,

Affiliation Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯
Miguel Alcalde

* E-mail: malcalde@icp.csic.es

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
⨯

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez,
Patricia Molina-Espeja,
Eva Garcia-Ruiz,
Miguel Alcalde

Published: March 10, 2014
https://doi.org/10.1371/journal.pone.0090919

About the Authors

David Gonzalez-Perez

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain

Patricia Molina-Espeja

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain

Eva Garcia-Ruiz

Affiliation Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America

Miguel Alcalde

* E-mail: malcalde@icp.csic.es

Affiliation Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MA. Performed the experiments: DGP PME EGR. Analyzed the data: DGP PME EGR MA. Contributed reagents/materials/analysis tools: PME EGR DGP. Wrote the paper: MA.

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Saccharomyces cerevisiae
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Polymerase chain reaction
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Yeast
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Signal peptides
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Peroxidases
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Mutagenesis
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Directed evolution
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Polymerases
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