Leveraging mathematical modeling framework to guide regimen strategy for phage therapy
Fig 4
In vitro and in silico time-kill kinetics.
(A-C) In vitro curves (blue) of phages (A) LUZ19, (B) PYO2, and (C) E215 treatment at MOI 1. Data shown as mean ± standard error of mean (SEM) of replicate wells (n>11). The curves flatten at the limit of detection (LOD) OD600 ~0.2. The logistic growth rate of phage infected cells BI (orange) and resistant cells BR (green) were fitted according to Eq 1. 95% confidence interval of model fitting is included as shaded area. (D-F) In vitro growth curve (blue) of cocktails (D) LUZ19+PYO2, (E) LUZ19+E215, or (F) PYO2+E215. Logistic growth rate of phage infected cells BI (orange), single-mutant resistant cells BR1 and BR2 (green), and double-mutant resistant cells BR12 (red) were fitted according to Eq 4 (D-E) and 5 (F).