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Vector construction  
PLOS One  
Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast

Danguole Ziogiene, Andrius Burdulis, Albertas Timinskas, Ruta Zinkeviciute, Emilija Vasiliunaite, Milda Norkiene, Alma Gedvilaite

Research Article | published 07 Nov 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0313330

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PPARgamma dependent PEX11beta counteracts the suppressive role of SIRT1 on neural differentiation of HESCs

Maryam Esmaeili, Mohammad Hossein Nasr-Esfahani, Alireza Shoaraye Nejati, Zahra Safaeinejad, Atefeh Atefi, Timothy L. Megraw, Kamran Ghaedi

Research Article | published 16 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0298274

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A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes

Shaun Ferguson, Nikolaj B. Abel, Dugald Reid, Lene H. Madsen, Thi-Bich Luu, Kasper R. Andersen, Jens Stougaard, Simona Radutoiu

Lab Protocol | published 01 Nov 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0291680

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Improved USER cloning for TALE assembly and its application to base editing

Jizeng Zhou, Jiaowei Wang, Fangbing Chen, Zhenpeng Zhuang, Min Chen, Yang Yang, Xian Luo, Chengcheng Tang, Xiaoqing Zhou, Yue Chi, Jinling Wang, Yu He, Kun Zhang, Qingjian Zou

Research Article | published 04 Aug 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0289509

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Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, Endang M. Septiningsih

Research Article | published 05 Apr 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0283837

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Bombyx mori miR-2845 represses the expression of fibroin light chain gene both in vitro and in vivo

JingYi Huang, YanHua Chen, Juan Zhu, MeiXian Wang, ShunMing Tang, YongZhu Yi, XingJia Shen

Research Article | published 16 Dec 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0261391

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Gibberellin induced transcription factor bZIP53 regulates CesA1 expression in maize kernels

Huayang Lv, Xiao Li, Hui Li, Yufeng Hu, Hanmei Liu, Shengjuan Wen, Yangping Li, Yinghong Liu, Huanhuan Huang, Guowu Yu, Yubi Huang, Junjie Zhang

Research Article | published 17 Mar 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0244591

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A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, Martina Fatho, Michael Volkmar, Roland Conradi, Matthias Theobald, Thomas Wölfel, Catherine Wölfel

Research Article | published 09 Sep 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0238875

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A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology

Sho Yoshimatsu, Takefumi Sone, Mayutaka Nakajima, Tsukika Sato, Ryotaro Okochi, Mitsuru Ishikawa, Mari Nakamura, Erika Sasaki, Seiji Shiozawa, Hideyuki Okano

Research Article | published 27 Aug 2019 PLOS ONE

https://doi.org/10.1371/journal.pone.0221164

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Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

Shigeo S. Sugano, Ryuichi Nishihama, Makoto Shirakawa, Junpei Takagi, Yoriko Matsuda, Sakiko Ishida, Tomoo Shimada, Ikuko Hara-Nishimura, Keishi Osakabe, Takayuki Kohchi

Research Article | published 31 Oct 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0205117

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Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha

Shoji Mano, Ryuichi Nishihama, Sakiko Ishida, Kazumi Hikino, Maki Kondo, Mikio Nishimura, Katsuyuki T. Yamato, Takayuki Kohchi, Tsuyoshi Nakagawa

Research Article | published 04 Oct 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0204964

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Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins

Masayuki Nakamura, Yuino Yasukawa, Akira Furusawa, Tamao Fuchiwaki, Takashi Honda, Yuta Okamura, Kiyotaka Fujita, Hisashi Iwai

Research Article | published 09 Aug 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0201982

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Magnolol-mediated regulation of plasma triglyceride through affecting lipoprotein lipase activity in apolipoprotein A5 knock-in mice

Chun-Kai Chang, Xiu-Ru Lin, Yen-Lin Lin, Woei-Horng Fang, Shu-Wha Lin, Sui-Yuan Chang, Jau-Tsuen Kao

Research Article | published 09 Feb 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0192740

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Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants

Tjaša Lukan, Fabian Machens, Anna Coll, Špela Baebler, Katrin Messerschmidt, Kristina Gruden

Research Article | published 04 Jan 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0190526

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Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

Andreas I. Andreou, Naomi Nakayama

Research Article | published 02 Jan 2018 PLOS ONE

https://doi.org/10.1371/journal.pone.0189892

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