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Ribonucleoproteins  
PLOS One  
A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

Lab Protocol | published 27 Mar 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0312722

Views: 77Citations: Saves: 0 • Shares: 0

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen, Kory Melton, Weng In Leong, Prachi Khekare, Shannon McCawley, Jean Chan, Pierre Boivin, Vihasi Jani, Aaron J. Cantor, Akshay Tambe, Mary Haak-Frendscho, Mary J. Janatpour, Spencer C. Wei

Research Article | published 24 Feb 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0317745

Views: 360Citations: 0Saves: 0 • Shares: 0

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Lab Protocol | published 14 Nov 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0310368

Views: 1167Citations: 1Saves: 0 • Shares: 0

Mechanisms of active diffusion of vesicular stomatitis virus inclusion bodies and cellular early endosomes in the cytoplasm of mammalian cells

Steven J. Moran, Ryan Oglietti, Kathleen C. Smith, Jed C. Macosko, George Holzwarth, Douglas S. Lyles

Research Article | published 14 Mar 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0290672

Views: 688Citations: 1Saves: 5 • Shares: 1

Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice

Daniel A. Kuppers, Jonathan Linton, Sergio Ortiz Espinosa, Kelly M. McKenna, Anthony Rongvaux, Patrick J. Paddison

Lab Protocol | published 28 Jun 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0287052

Views: 1777Citations: 3Saves: 0 • Shares: 0

A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors

Koollawat Chupradit, Nontaphat Thongsin, Chatchai Tayapiwatana, Methichit Wattanapanitch

Lab Protocol | published 07 Jul 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0270963

Views: 3061Citations: 3Saves: 13 • Shares: 4

Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles

Andrew R. Castle, Serene Wohlgemuth, Luis Arce, David Westaway

Research Article | published 07 Jun 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0269342

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Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum

Nahyun Lee, Jiyeun Park, Jung-Eun Kim, Ji Young Shin, Kyunghun Min, Hokyoung Son

Research Article | published 03 Jun 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0268855

Views: 4777Citations: 7Saves: 19 • Shares: 1

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin

Study Protocol | published 09 Nov 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0259812

Views: 7870Citations: 21Saves: 49 • Shares: 2

Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing

Eric A. Noel, Donald P. Weeks, James L. Van Etten

Research Article | published 21 Oct 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0252696

Views: 1750Citations: 5Saves: 0 • Shares: 0

Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach

Nela Pavlikova, Jan Sramek, Michael Jelinek, Petr Halada, Jan Kovar

Research Article | published 26 Oct 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0229430

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Interaction between influenza A virus nucleoprotein and PB2 cap-binding domain is mediated by RNA

Wun-Chung Szeto, Ho-Pan Hsia, Yun-Sang Tang, Pang-Chui Shaw

Research Article | published 28 Sep 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0239899

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Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay

Pin Lyu, Kyung Whan Yoo, Manish Kumar Yadav, Anthony Atala, Annemieke Aartsma-Rus, Maaike van Putten, Dongsheng Duan, Baisong Lu

Research Article | published 24 Sep 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0239468

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Evolutionary and biochemical analyses reveal conservation of the Brassicaceae telomerase ribonucleoprotein complex

Kelly Dew-Budd, Julie Cheung, Kyle Palos, Evan S. Forsythe, Mark A. Beilstein

Research Article | published 09 Apr 2020 PLOS ONE

https://doi.org/10.1371/journal.pone.0222687

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Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes

Vikas Kumar, Pawan K. Doharey, Shelly Gulati, Joshua Meehan, Mary G. Martinez, Karrisa Hughes, Blaine H. M. Mooers, Jorge Cruz-Reyes

Research Article | published 29 Apr 2019 PLOS ONE

https://doi.org/10.1371/journal.pone.0211525

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