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RNA extraction  
PLOS One  
mRNA extracted from frozen buffy coat samples stored long term in tubes with no RNA preservative shows promise for downstream sequencing analyses

Erik Bovinder Ylitalo, Linda Vidman, Sophia Harlid, Bethany Van Guelpen

Research Article | published 19 Mar 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0318834

Views: 227Citations: 0Saves: 0 • Shares: 0

Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques

Simona Spiteri, Irene Salamon, Luna Girolamini, Maria Rosaria Pascale, Federica Marino, Carlo Derelitto, Laura Caligaris, Simone Paghera, Manuela Ferracin, Sandra Cristino

Research Article | published 03 Feb 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0317228

Views: 505Citations: 0Saves: 0 • Shares: 0

Detection of SARS-CoV-2 and a possible variant in shelter cats

Ogi Okwumabua, Nancy Bradley-Siemens, Catherine Cruz, Lauren Chittick, Melissa Thompson

Research Article | published 13 Jan 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0317104

Views: 449Citations: 0Saves: 3 • Shares: 1

Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens

Nkechi Martina Odogwu, Jin Sung Jang, Sabrina Albertson, Clinton Hagen, Boyd Rasmussen, Oommen Saji, Timothy J. Nelson, the Wanek Program Preclinical Pipeline

Research Article | published 26 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0315098

Views: 387Citations: 0Saves: 0 • Shares: 0

Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis

Marc Van Der Hofstadt, Anna Cardinal, Morgane Lepeltier, Jérémy Boulestreau, Alimata Ouedraogo, Malik Kahli, Pierre Champigneux, Laurence Molina, Franck Molina, Thi Nhu Ngoc Van

Research Article | published 10 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0314733

Views: 778Citations: 1Saves: 0 • Shares: 0

Exploring the diagnostic potential of miRNA signatures in the Fabry disease serum: A comparative study of automated and manual sample isolations

Josephine Y. Fang, Saravanan Ayyadurai, Alyssa F. Pybus, Hiroshi Sugimoto, Mark G. Qian

Research Article | published 28 Oct 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0301733

Views: 590Citations: 1Saves: 0 • Shares: 0

A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus

Phostine M. Kirasi, Elijah M. Ateka, Edith K. Avedi, Hillary K. Yegon, Bramwel W. Wanjala, Hanu R. Pappu

Research Article | published 13 Jun 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0304497

Views: 1209Citations: 1Saves: 2 • Shares: 2

Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1

Chunlin Peng, Benyi Fan, Hui Zhu, Liyun Liu, Zhengwu Zhao, Liyun Huang

Research Article | published 05 Jun 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0303941

Views: 518Citations: 0Saves: 4 • Shares: 1

Evaluation of short-term hair follicle storage conditions for maintenance of RNA integrity

Eilís E. Harkin, John A. Browne, Barbara A. Murphy

Research Article | published 31 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0294089

Views: 568Citations: 0Saves: 0 • Shares: 0

Efficient wastewater sample filtration improves the detection of SARS-CoV-2 variants: An extensive analysis based on sequencing parameters

Angelo Robotto, Carlotta Olivero, Elisa Pozzi, Claudia Strumia, Camilla Crasà, Cristina Fedele, Maddalena Derosa, Massimo Di Martino, Stefania Latino, Giada Scorza, Andrea Civra, David Lembo, Paola Quaglino, Enrico Brizio, Denis Polato

Research Article | published 24 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0304158

Views: 817Citations: 0Saves: 4 • Shares: 4

Genetic diversity and distribution of noroviruses among all age groups of patients with diarrhea in Amhara National Regional State, Ethiopia

Dessie Tegegne, Aschalew Gelaw, Dawit Hailu Alemayehu, Tamrayehu Seyoum, Dereje Leta, Getachew Ferede, Andargachew Mulu, Baye Gelaw

Research Article | published 21 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0303887

Views: 708Citations: 0Saves: 26 • Shares: 1

Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel, Wesley A. Tom, Chao Jiang, Gary Krzyzanowski, Nirmalee Fernando, Appolinaire Olou, M. Rohan Fernando

Research Article | published 20 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0303598

Views: 748Citations: 0Saves: 10 • Shares: 0

Performance of amplicon and capture based next-generation sequencing approaches for the epidemiological surveillance of Omicron SARS-CoV-2 and other variants of concern

Carlos Daviña-Núñez, Sonia Pérez, Jorge Julio Cabrera-Alvargonzález, Anniris Rincón-Quintero, Ana Treinta-Álvarez, Montse Godoy-Diz, Silvia Suárez-Luque, Benito Regueiro-García

Research Article | published 29 Apr 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0289188

Views: 850Citations: 1Saves: 6 • Shares: 1

Phytophthora pluvialis maintenance, spore production and detached needle assays

Sophie Eccersall, Leann S. Vinson, Rebecca McDougal, Claudia-Nicole Meisrimler

Lab Protocol | published 21 Mar 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0293817

Views: 675Citations: 0Saves: 5 • Shares: 1

A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples

Sofia Levin Nikulin, Poppy J. Hesketh-Best, Dean A. Mckeown, Marla Spivak, Declan C. Schroeder

Research Article | published 14 Mar 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0297623

Views: 1371Citations: 5Saves: 9 • Shares: 3

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