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Gene targeting  
PLOS One  
Development of a lambda Red based system for gene deletion in Chlamydia

Yibing Wang, Robert Suchland, Amy Hua, Steven Carrell, Daniel Rockey, Kevin Hybiske

Research Article | published 14 Nov 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0311630

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Non-replicative phage particles delivering CRISPR-Cas9 to target major blaCTX-M variants

Naiyaphat Nittayasut, Teerapong Yata, Sunisa Chirakul, Navapon Techakriengkrai, Pattrarat Chanchaithong

Research Article | published 16 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0303555

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Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach

Umi Nuraeni, Jekmal Malau, Retno Tri Astuti, Auraga Dewantoro, Dini Apriori, Evellin Dewi Lusiana, Bambang Prasetya

Research Article | published 14 Jul 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0287712

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Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

Research Article | published 24 Feb 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0279431

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Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

Research Article | published 20 Jan 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0280894

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Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4

Shuang Li, Yichao Tang, Lingran Tang, Xuanyu Yan, Jiali Xiao, Huijun Xiang, Qing Wu, Ruqi Yu, Yushi Jin, Jingyu Yu, Nuo Xu, Chu Wu, Shengqin Wang, Chuanhua Wang, Qiongzhen Chen

Research Article | published 02 Dec 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0278503

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The deletion of HK-1 gene affects the bacterial virulence of Pseudomonas stutzeri LH-42

Si Shan, Tingting Hu, Yu Yang

Research Article | published 29 Nov 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0277089

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High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

Research Article | published 09 Sep 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0273873

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Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

Tate Oulton, Joshua Obiero, Isabel Rodriguez, Isaac Ssewanyana, Rebecca A. Dabbs, Christine M. Bachman, Bryan Greenhouse, Chris Drakeley, Phil L. Felgner, Will Stone, Kevin K. A. Tetteh

Research Article | published 29 Aug 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0273106

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Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum

Nahyun Lee, Jiyeun Park, Jung-Eun Kim, Ji Young Shin, Kyunghun Min, Hokyoung Son

Research Article | published 03 Jun 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0268855

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Identification of miRNA signature for predicting the prognostic biomarker of squamous cell lung carcinoma

Huanqing Liu, Tingting Li, Chunsheng Dong, Jun Lyu

Research Article | published 15 Mar 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0264645

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Founder transformants of cotton (Gossypium hirsutum L.) obtained through the introduction of DS-Red, Rec, Rep and CRISPR/Cas9 expressing constructs for developing base lines of recombinase mediated gene stacking

Sabin Aslam, Sultan Habibullah Khan, Aftab Ahmad, Sriema Lalani Walawage, Abhaya M. Dandekar

Research Article | published 03 Feb 2022 PLOS ONE

https://doi.org/10.1371/journal.pone.0263219

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Characterization of Poldip2 knockout mice: Avoiding incorrect gene targeting

Bernard Lassègue, Sandeep Kumar, Rohan Mandavilli, Keke Wang, Michelle Tsai, Dong-Won Kang, Catherine Demos, Marina S. Hernandes, Alejandra San Martín, W. Robert Taylor, Hanjoong Jo, Kathy K. Griendling

Research Article | published 20 Dec 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0247261

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Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system

Worarat Kruasuwan, Aekkachai Puseenam, Chitwadee Phithakrotchanakoon, Sutipa Tanapongpipat, Niran Roongsawang

Research Article | published 28 Sep 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0258005

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Flow Assisted Mutation Enrichment (FAME): A highly efficacious and efficient method to enrich Double Knockouts (DKO) after gene editing

Michael Hansen, Xiaopin Cai, Sara Bowen, David A. Largaespada, Ming V. Li

Research Article | published 04 Mar 2021 PLOS ONE

https://doi.org/10.1371/journal.pone.0247375

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