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A protocol for loading Calcein-AM into extracellular vesicles from mammalian cells for clear visualization with a fluorescence microscope coupled to a deconvolution system

María-Angélica Calderón-Peláez, Jaime E. Castellanos, Myriam L. Velandia-Romero

Lab Protocol | published 24 Jan 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0317689

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AptaFluorescence: An aptamer-based fluorescent imaging protocol for biomolecule visualization

Crystal Chia Yin Ling, Melissa Jane Fullwood

Research Article | published 27 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0316359

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Development of a molecular genetics and cell biology toolbox for the filamentous fungus Diplodia sapinea

Anne Geertje Oostlander, Laura Brodde, Miriam von Bargen, Bernard Slippers, Yvonne Becker, Ulrike Brandt, Frank Klawonn, Christiaan Grobler, Lucas Well, Jan Stenlid, Jonas Oliva, Malin Elfstrand, Andre Fleissner

Lab Protocol | published 27 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0308794

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Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study

Pavel Fikar, Laura Alvarez, Laura Berne, Martin Cienciala, Christopher Kan, Hynek Kasl, Mona Luo, Zuzana Novackova, Sheyla Ordonez, Zuzana Sramkova, Monika Holubova, Daniel Lysak, Lyndsay Avery, Andres A. Caro, Roslyn N. Crowder, Laura A. Diaz-Martinez, David W. Donley, Rebecca R. Giorno, Irene K. Guttilla Reed, Lori L. Hensley, Kristen C. Johnson, Audrey Y. Kim, Paul Kim, Adriana J. LaGier, Jamie J. Newman, Elizabeth Padilla-Crespo, Nathan S. Reyna, Nikolaos Tsotakos, Noha N. Al-Saadi, Tayler Appleton, Ana Arosemena-Pickett, Braden A. Bell, Grace Bing, Bre Bishop, Christa Forde, Michael J. Foster, Kassidy Gray, Bennett L. Hasley, Kennedy Johnson, Destiny J. Jones, Allison C. LaShall, Kennedy McGuire, Naomi McNaughton, Angelina M. Morgan, Lucas Norris, Landon A. Ossman, Paollette A. Rivera-Torres, Madeline E. Robison, Kathryn Thibodaux, Lescia Valmond, Daniel Georgiev

Research Article | published 09 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0314992

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Platelet activation near point-like source of agonist: Experimental insights and computational model

Ezhena S. Starodubtseva, Tatyana Yu. Karogodina, Alexander E. Moskalensky

Research Article | published 03 Oct 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0308679

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Coumarin derivatives as new anti-biofilm agents against Staphylococcus aureus

Atia-tul- Wahab, Faiza Nadeem, Uzma Salar, Hafiz Muhammad Bilal, Mehak Farooqui, Sumaira Javaid, Sohira Sadaf, Khalid M. Khan, M. Iqbal Choudhary

Research Article | published 19 Sep 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0307439

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Prevalence of pulmonary tuberculosis among key and vulnerable populations in hotspot settings of Ethiopia. A systematic review and meta-analysis

Melese Abate Reta, Zelalem Asmare, Assefa Sisay, Yalewayker Gashaw, Ermias Getachew, Muluken Gashaw, Zelalem Dejazmach, Abdu Jemal, Solomon Gedfie, Getinet Kumie, Marye Nigatie, Wagaw Abebe, Agenagnew Ashagre, Tadesse Misganaw, Woldeteklehaymanot Kassahun, Selamyhun Tadesse, Alene Geteneh, Atitegeb Abera Kidie, Biruk Beletew Abate, Nontuthuko Excellent Maningi, P. Bernard Fourie

Research Article | published 29 Aug 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0309445

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An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence