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DNA libraries  
PLOS One  
A method for PCR-free library preparation for sequencing palaeogenomes

Kirstin Henneberger, Axel Barlow, Federica Alberti, Michaela Preick, Silviu Constantin, Doris Döppes, Wilfried Rosendahl, Michael Hofreiter, Johanna L. A. Paijmans

Research Article | published 19 Mar 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0319573

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Error-corrected ultradeep next-generation sequencing for detection of clonal haematopoiesis and haematological neoplasms – sensitivity, specificity and accuracy

Melinda L. Tursky, Crisbel M. Artuz, Melissa Rapadas, Gary A. Wittert, Timothy J. Molloy, David D. Ma

Research Article | published 26 Feb 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0318300

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A new method for the reproducible development of aptamers (Neomers)

Cathal Meehan, Erika L. Hamilton, Chloé G. Mansour, Soizic Lecocq, Cole J. Drake, Yisi An, Emily Rodrigues, Gregory Penner

Research Article | published 12 Feb 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0311497

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Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide

Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

Research Article | published 09 Jan 2025 PLOS ONE

https://doi.org/10.1371/journal.pone.0313009

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Formalin-free tissue embedding is less hazardous and results in better DNA quality

Maarten Niemantsverdriet, Ageeth Knol, Jose van der Starre-Gaal, Agnes Marije Hoogland

Research Article | published 30 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0316107

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RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, Katherine Lauro, Vasudha Nimmagadda, Quynh Tong, Kayla Bello, Daniel Woldring, Masako Harada

Research Article | published 02 Dec 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0312188

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Comparative analysis of whole exome sequencing kits for the canine genome

Jinhee Jang, Yong-Jik Lee, Soohyun Ko, A. M. Abd El-Aty, Ibrahim Gecili, Ji Hoon Jeong, ChangHyuk Kwon, Tae Woo Jung

Research Article | published 04 Nov 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0312203

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Spatial transcriptomic profiling of isolated microregions in tissue sections utilizing laser-induced forward transfer

Kaiqiang Ye, Wanqing Chang, Jitao Xu, Yunxia Guo, Qingyang Qin, Kaitong Dang, Xiaofeng Han, Xiaolei Zhu, Qinyu Ge, Qiannan Cui, Yun Xu, Xiangwei Zhao

Research Article | published 25 Jul 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0305977

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A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, Tsutomu Hosouchi, Takashi Watanabe, Izumi Yamaguchi, Manabu Nakayama, Osamu Ohara

Research Article | published 05 Jul 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0302451

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Exploring the potential of Oxford Nanopore Technologies sequencing for Mycobacterium tuberculosis sequencing: An assessment of R10 flowcells and V14 chemistry

Anzaan Dippenaar, Emilyn Costa Conceição, Felicia Wells, Johannes Loubser, Brendon Mann, Miguel De Diego Fuertes, Vincent Rennie, Robin Mark Warren, Annelies Van Rie

Research Article | published 06 Jun 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0303938

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Improving DNA barcoding library of armored scale insects (Hemiptera: Diaspididae) in China

Minmin Niu, Yubo Liu, Linjia Xue, Bo Cai, Qing Zhao, Jiufeng Wei

Research Article | published 30 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0301499

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NGS method for parallel processing of high quality, damaged or fragmented input material using target enrichment

Anna Lyander, Anna Gellerbring, Moa Hägglund, Keyvan Elhami, Valtteri Wirta

Research Article | published 29 May 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0304411

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High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing

Thomas Bygh Nymann Jensen, Sebastian Mølvang Dall, Simon Knutsson, Søren Michael Karst, Mads Albertsen

Research Article | published 04 Apr 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0301446

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High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method

Lauren Cobb, Erik de Muinck, Spyros Kollias, Morten Skage, Gregor D. Gilfillan, Markus A. K. Sydenham, Shuo-Wang Qiao, Bastiaan Star

Research Article | published 22 Mar 2024 PLOS ONE

https://doi.org/10.1371/journal.pone.0300865

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Adaptation of CUT&RUN for use in African trypanosomes

Geneva Miller, Lindsey M. Rollosson, Carrie Saada, Serenity J. Wade, Danae Schulz

Lab Protocol | published 21 Nov 2023 PLOS ONE

https://doi.org/10.1371/journal.pone.0292784

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