Gap Junctional Communication in Osteocytes Is Amplified by Low Intensity Vibrations In Vitro
Figure 4
Vibration induced GJIC is controlled by Akt signaling.
One hour after vibration treatment, (A) Cx43 mRNA levels remained unchanged. (B) Cx43 protein levels were also unaffected by vibrations (0.15 g–100 Hz) without and with Akt inhibition but (C) vibrations increased Akt phosphorylation (ser 473) 2.4-fold. (D) Inhibition of Akt activation also inhibited the vibration induced increase in calcein transference. ***: p<0.001 against control.