The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury
Fig 3
Optimal dose of Domain V in cardiac IRI.
(A) C57BL/6 WT mice were treated with control or escalating doses of rhDomain V (n = 7) and assayed for troponin I by ELISA. (B) Mice treated with rhDomain V 40 μM (n = 7) had lower levels of apoptosis compared with control (n = 7). (C) Representative image from within the AAR showing apoptotic cells using the Deadend Fluorometric TUNEL system in a mouse treated with sham LAD ligation, (D) saline and (E) rh Domain V. Images obtained using a Zeiss AxioVert A1 light microscope. DV = Domain V. Points represent number of apoptotic cells in individual mouse cardiac tissue. The horizontal line for each group represents the mean.
