A Balance between Nuclear and Cytoplasmic Volumes Controls Spindle Length
Fig 3
Nuclear to cytoplasmic ratio in cells created by fusion, enucleation, or parthenogenetic activation.
Panel A: The chart shows nuclear to cytoplasmic ratio in all cells used in experiments described in Figs 1 and 2: Intact cells (0.046, n = 93), 2 fused cells (0.047, n = 28), 3 fused cells (0.048, n = 13), 2 fused enucleated cells (0.027, n = 12) and unfertilized cells (0.043, n = 40). N:C ratio of Intact cells is significantly different in comparison to 2 fused enucleated (p < 0.0001) and unfertilized cells (p = 0.0116). The diagram below the X-axis illustrates the proportional differences between cell and nuclear size of each cell type. Panel B: The plot shows a relationship between nuclear to cytoplasmic ratio and spindle length in all cells used in experiments described in Figs 1 and 2: Green dots represent intact cells (spindle length: 32.75 ± 4.30; NC ratio: 0.046 ± 0.006), red squares represent 2 fused cells (spindle length: 35.11 ± 4.49; NC ratio: 0.047 ± 0.008), orange triangles represent 3 fused cells (spindle length: 40.70 ± 3.56; NC ratio: 0.048 ± 0.003), blue rhombuses represent unfertilized cells (spindle length: 38.04 ± 4.06; NC ratio: 0.043 ± 0.006) and gray hexagons represent 2 fused enucleated cells (spindle length: 43.20 ± 3.69; NC ratio: 0.027 ± 0.003).
